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Product Name
Rat Ephrin B3 / EFNB3 (Fc Tag) recombinant protein
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Description
Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons.
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Protein name
Ephrin B3 (Predicted)
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Protein short names
EPLG8; EPHRIN B3 / EFNB3; RP23-56I20.5; ELF-3; EPL8; LERK-8; EPH-RELATED RECEPTOR TRANSMEMBRANE LIGAND ELK-L3; ELK-L3; EFL-6; LERK8; EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 8; EFL6; EPHRIN-B3; NLERK-2
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Uniprot ID
G3V7D4
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Gene Name
Efnb3; Efnb3_predicted; rCG_32839
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the rat EFNB3 (NP_001094450) (Met1-Ser224) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
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Protein Species
Rat
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Molecular weight
The recombinant rat EFNB3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 438 amino acids and has a predicted molecular mass of 48.7 kDa. The apparent molecular mass of the protein is approximately 55 and 34 kDa in SDS-PAGE under reducing conditions.
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Purity
(79.6+17) % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized mouse EphB3-His (Cat:500494) at 10 μg/ml (100 μl/well) can bind rat EFNB3-Fc, The EC50 of rat EFNB3-Fc is 14.1-33.2 ng/ml. -
Validations
Rat Ephrin-B3 / EFNB3 Protein (Fc Tag) SDS-PAGE
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