EIF4A1 Polyclonal Antibody

Cat.#: 164470

Special Price 174.2 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    EIF4A1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to EIF4A1
  • Tested applications
    WB, IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    EIF4A1 antibody; DDX2A antibody; EIF-4A antibody; EIF4A antibody; eIF-4A-I antibody; eIF4A-I antibody; eukaryotic initiation factor 4A-I antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:20 - 1:50
    IP 1:20 - 1:50
  • Validations

    Western blot - EIF4A1 Polyclonal Antibody

    Western blot - EIF4A1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using EIF4A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunofluorescence - EIF4A1 Polyclonal Antibody

    Immunofluorescence - EIF4A1 Polyclonal Antibody

    Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody . Blue: DAPI for nuclear staining.

  • Background
    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"