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Product Name
EIF4A1 Polyclonal Antibody
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Description
Polyclonal antibody to EIF4A1
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
EIF4A1 antibody; DDX2A antibody; EIF-4A antibody; EIF4A antibody; eIF-4A-I antibody; eIF4A-I antibody; eukaryotic initiation factor 4A-I antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:50
IP 1:20 - 1:50 -
Validations
Western blot - EIF4A1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF4A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - EIF4A1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody . Blue: DAPI for nuclear staining.
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Background
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"