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Anti-eIF4A1 (6D7) Mouse antibody

Cat.#: 168099

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-eIF4A1 (6D7) Mouse antibody
  • Documents
  • Description
    eIF4A1 (6D7) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Synthetic Peptide of eIF4A1
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000-3000

    IF: 1/100-200

    IHC: 1/50-300

  • Validations

    IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100

    IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100

    Western blot detection of eIF4A1 in T47D,3T3,COS7,C6 and Hela cell lysates using eIF4A1 mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.

    Western blot detection of eIF4A1 in T47D,3T3,COS7,C6 and Hela cell lysates using eIF4A1 mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.

  • Background
    Swiss-Prot Acc.P60842.ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"