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Product Name
EIF3M antibody
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Description
EIF3M Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in mouse uterus tissue, HeLa cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EIF3M antibody; Fetal lung protein B5 antibody; FLJ29030 antibody; GA17 antibody; hfl B5 antibody; HFLB5 antibody; PCID1 antibody; PNAS 125 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF3M recombinant protein (Accession Number: NM_006360). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse uterus tissue were subjected to SDS PAGE followed by western blot with Catalog No:110200(EIF3M antibody) at dilution of 1:500
IP Result of anti-EIF3M (IP:Catalog No:110200, 3ug; Detection:Catalog No:110200 1:800) with HeLa cells lysate 3800ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110200(EIF3M antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using EIF3M antibody Catalog No:110200 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).
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Background
EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.
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References
- Goh SH, Hong SH, Hong SH. eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer. Oncogene. 30(4):398-409. 2011.
- Chew SK, Chen P, Link N, Galindo KA, Pogue K, Abrams JM. Genome-wide silencing in Drosophila captures conserved apoptotic effectors. Nature. 460(7251):123-7. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"