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Product Name
EIF3M antibody
- Documents
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Description
EIF3M Mouse Monoclonal antibody. Positive WB detected in Hela cells, HepG2 cells, MCF7 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 43kD
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
EIF3M antibody; Fetal lung protein B5 antibody; FLJ29030 antibody; GA17 antibody; hfl B5 antibody; HFLB5 antibody; PCID1 antibody; PNAS 125 antibody
- Immunogen
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of EIF3M recombinant protein (Accession Number: NM_006360). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107252(EIF3M antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using EIF3M antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"