ECH1 antibody

Cat.#: 110292

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Product Information

  • Product Name
    ECH1 antibody
  • Documents
  • Description
    ECH1 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse heart tissue. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30-36kd
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ECH1 antibody; HPXEL antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ECH1 recombinant protein (Accession Number: NM_001398). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110292(ECH1 antibody) at dilution of 1:500

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110292(ECH1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110292(ECH1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110292(ECH1 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using ECH1 antibody Catalog No:110292 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using ECH1 antibody Catalog No:110292 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    ECH1 represents enoyl Coenzyme A hydratase 1, peroxisomal
  • References
    • Ishimoto T, Lanaspa MA, Rivard CJ. High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase. Hepatology (Baltimore, Md.). 58(5):1632-43. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"