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Product Name
Anti-ECH1 antibody
- Documents
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Description
Rabbit Monoclonal to Human ECH1
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Tested applications
WB, ELISA
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Species reactivity
Human ECH1
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Alternative names
HPXEL antibody; AA617331 antibody; enoyl CoA hydratase 1 antibody; peroxisomal antibody; ECH1 antibody; HPXEL antibody; delta(3 antibody; 5)-Delta(2 antibody; 4)-dienoyl-CoA isomerase antibody; mitochondrial antibody; delta3 antibody; 5-delta2 antibody; 4-dienoyl-CoA isomerase antibody; enoyl Coenzyme A hydratase 1 antibody; peroxisomal antibody; peroxisomal enoyl-CoA hydratase 1 antibody; enoyl coenzyme A hydratase 1 antibody; peroxisomal antibody; Ech1 antibody; AA617331 antibody; delta(3 antibody; 5)-Delta(2 antibody; 4)-dienoyl-CoA isomerase antibody; mitochondrial antibody; dienoyl-CoA isomerase antibody; peroxisomal/mitochondrial dienoyl-CoA isomerase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ECH1 .
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-5 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human ECH1.
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Validations
ECH1 Antibody, Rabbit MAb, Western blot
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Background
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"