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Product Name
Anti-ECH1 antibody
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Description
Rabbit polyclonal to ECH1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human ECH1
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Alternative names
HPXEL antibody; AA617331 antibody; enoyl CoA hydratase 1 antibody; peroxisomal antibody; ECH1 antibody; HPXEL antibody; delta(3 antibody; 5)-Delta(2 antibody; 4)-dienoyl-CoA isomerase antibody; mitochondrial antibody; delta3 antibody; 5-delta2 antibody; 4-dienoyl-CoA isomerase antibody; enoyl Coenzyme A hydratase 1 antibody; peroxisomal antibody; peroxisomal enoyl-CoA hydratase 1 antibody; enoyl coenzyme A hydratase 1 antibody; peroxisomal antibody; Ech1 antibody; AA617331 antibody; delta(3 antibody; 5)-Delta(2 antibody; 4)-dienoyl-CoA isomerase antibody; mitochondrial antibody; dienoyl-CoA isomerase antibody; peroxisomal/mitochondrial dienoyl-CoA isomerase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ECH1 (rh ECH1; Q13011; Thr34-Leu328). ECH1 specific IgG was purified by Human ECH1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ECH1. The detection limit for Human ECH1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 0.5-2 μg/mg of lysate
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Validations
ECH1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
ECH1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ECH1 in human liver(cirrhosis) with rabbit polyclonal antibody (0.1µg/mL, formalin-fixed paraffin embedded sections).Positive staining was localized to cytoplasm of hepatocyte
ECH1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ECH1 in human prostate with rabbit polyclonal antibody (0.1µg/mL, formalin-fixed paraffin embedded sections).
ECH1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
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References
- Kovalyov LI, et al. (2006) Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53.
- Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48.
- FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"