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Product Name
DNAJB6 Polyclonal Antibody
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Description
Polyclonal antibody to DNAJB6
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DNAJB6 antibody; DJ4 antibody; DnaJ antibody; HHDJ1 antibody; HSJ-2 antibody; HSJ2 antibody; LGMD1D antibody; LGMD1E antibody; MRJ antibody; MSJ-1 antibody; dnaJ homolog subfamily B member 6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - DNAJB6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DNAJB6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - DNAJB6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DNAJB6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DNAJB6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DNAJB6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DNAJB6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - DNAJB6 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using DNAJB6 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - DNAJB6 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using DNAJB6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"