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Product Name
DDB1 antibody
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Description
DDB1 Mouse Monoclonal antibody. Positive IHC detected in human cervical cancer tissue, human testis tissue. Positive IF detected in Hela cells, MCF-7 cells. Positive WB detected in Hela cells, A549 cells, MCF7 cells, NIH/3T3 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 127 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DDB p127 subunit antibody; DDB1 antibody; DDBA antibody; DNA damage binding protein 1 antibody; DNA damage binding protein a antibody; HBV X assOCiated protein 1 antibody; UV damaged DNA binding factor antibody; UV DDB 1 antibody; UV DDB1 antibody; XAP 1 antibody; XAP1 antibody; XPCE antibody; XPE antibody; XPE BF antibody; XPE binding factor antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of DDB1 recombinant protein (Accession Number: NM_001923). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107237(DDB1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107237(DDB1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107237(DDB1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using DDB1 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.
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References
- Jáuregui P, Logue EC, Schultz ML, Fung S, Landau NR. Degradation of SAMHD1 by Vpx Is Independent of Uncoating. Journal of virology. 89(10):5701-13. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"