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Product Name
Cytokeratin 20 antibody
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Description
Cytokeratin 20 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human colon tissue, human appendix tissue, human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, rat bladder tissue, rat colon tissue. Positive WB detected in COLO 320 cells, HeLa cells. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CK 20 antibody; CK20 antibody; Cytokeratin 20 antibody; K20 antibody; keratin 20 antibody; KRT20 antibody; KRT21 antibody; Protein IT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cytokeratin 20 recombinant protein (Accession Number: NM_019010). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:109805(KRT20 antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109805(KRT20 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109805(KRT20 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:109805(KRT20 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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References
- Fogelgren B, Polgar N, Lui VH. Urothelial Defects from Targeted Inactivation of Exocyst Sec10 in Mice Cause Ureteropelvic Junction Obstructions. PloS one. 10(6):e0129346. 2015.
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