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Product Name
Cytokeratin 20 antibody
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Description
Cytokeratin 20 Mouse Monoclonal antibody. Positive IHC detected in human colon tissue, human colon cancer tissue, human urothelial carcinoma tissue. Positive WB detected in Colo320 cells, COLO 320 cells. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CK 20 antibody; CK20 antibody; Cytokeratin 20 antibody; K20 antibody; keratin 20 antibody; KRT20 antibody; KRT21 antibody; Protein IT antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Cytokeratin 20 recombinant protein (Accession Number: NM_019010). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107233(KRT20 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:107233(KRT20 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:107233(KRT20 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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