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Product Name
Cytoglobin antibody
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Description
Cytoglobin Mouse Monoclonal antibody. Positive IHC detected in human liver tissue, human bladder tissue, human heart tissue. Positive WB detected in Human heart, pig heart tissue, rat heart tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 27-29 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Pig, Rat; other species not tested.
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Alternative names
CYGB antibody; cytoglobin antibody; HGB antibody; Histoglobin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Cytoglobin recombinant protein (Accession Number: NM_134268). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107228(CYGB antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107228(CYGB antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107228(CYGB antibody) at dilution of 1:50 (under 40x lens)
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Background
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"