Human CDK4 (GST Tag) recombinant protein

Human CDK4 (GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product. CDK4 is the ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. CDK4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma.

Cyclin-dependent kinase 4, isoform CRA_a

CRK3; MGC14458; PSK-J3; CDK4; CMM3

P11802

CDK4; hCG_40151

Baculovirus-Insect Cells

A DNA sequence encoding the human CDK4 (NP_000066.1) (Met 1-Glu 303) was fused with the GST tag at the N-terminus.

Human

The recombinant human CDK4/GST chimera consists of 528 amino acids and predicts a molecular mass of 60 kDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

No Kinase Activity