Cart 2
  • Anti-Cdk4 (3F9) Mouse antibody
  • Anti-Cdk4 (3F9) Mouse antibody

Anti-Cdk4 (3F9) Mouse antibody

Cat.#: 168128

Special Price 123.9 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-Cdk4 (3F9) Mouse antibody
  • Documents
  • Description
    Cdk4 (3F9) Mouse monoclonal antibody
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    Cdk 4;cdk4;CDK4 protein;CDK4_HUMAN;Cell division kinase 4;Cell division protein kinase 4;CMM 3;CMM3;Crk3;Cyclin dependent kinase 4;Cyclin-dependent kinase 4;Melanoma cutaneous malignant 3;MGC14458;p34 cdk4;PSK J3;PSK-J3. antibody
  • Isotype
    IgG1
  • Preparation
    Antigen: Purified recombinant human CDK4 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal antibody
  • Formulation
    Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000;Other 1/1000

  • Validations

    Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

    Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

    Western blot detection of CDK4 in Hela,C6 and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.

    Western blot detection of CDK4 in Hela,C6 and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:34KDa.

  • Background
    Swiss-Prot Acc.P11802.Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • References
    • Metformin inhibits cell proliferation in SKM-1 cells via AMPK-mediated cell cycle arrest

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"