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  • Anti-Cdk4 Rabbit antibody
  • Anti-Cdk4 Rabbit antibody

Anti-Cdk4 Rabbit antibody

Cat.#: 167021

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-Cdk4 Rabbit antibody
  • Documents
  • Description
    Cdk4 Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    CMM3; PSK-J3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant protein of human Cdk4
  • Clonality
    Polyclonal antibody
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/1000

    IHC: 1/50

    ICC/IF: 1/50

    IP: 1/20

  • Validations

    Western blot detection of Cdk4 in K562,C6,3T3,Hela cell lysates using Cdk4 Rabbit pAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:34kDa.

    Western blot detection of Cdk4 in K562,C6,3T3,Hela cell lysates using Cdk4 Rabbit pAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:34kDa.

    Immunohistochemistry of Cdk4 in paraffin-embedded Human lung cancer tissue using Cdk4 Rabbit pAb at dilution 1/50

    Immunohistochemistry of Cdk4 in paraffin-embedded Human lung cancer tissue using Cdk4 Rabbit pAb at dilution 1/50

  • Background
    Swiss-Prot Acc.P11802.Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"