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Product Name
CSNK1A1 Polyclonal Antibody
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Description
Polyclonal antibody to CSNK1A1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CSNK1A1 antibody; CK1 antibody; CK1a antibody; CKIa antibody; HEL-S-77p antibody; HLCDGP1 antibody; PRO2975 antibody; casein kinase 1 alpha 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CSNK1A1 (NP_001020276.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CSNK1A1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CSNK1A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
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Background
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"