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Product Name
CSK antibody
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Description
CSK Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human lung tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue. Positive WB detected in HL-60 cells, Jurkat cells, K-562 cells, rat lung tissue. Positive IP detected in HL-60 cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
C Src kinase antibody; c src tyrosine kinase antibody; CSK antibody; Protein tyrosine kinase CYL antibody; Tyrosine protein kinase CSK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CSK recombinant protein (Accession Number: NM_001354988). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109597(CSK antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung using Catalog No:109597(CSK antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:109597(CSK antibody) at dilution of 1:200 (under 40x lens)
IP Result of anti-CSK (IP:Catalog No:109597, 4ug; Detection:Catalog No:109597 1:800) with HL-60 cells lysate 1000ug.
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:109597(CSK Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).
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References
- Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Rönnblom L. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. Journal of immunology (Baltimore, Md. : 1950). 191(6):2989-98. 2013.
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