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  • Anti-CSK (5E10) Mouse antibody
  • Anti-CSK (5E10) Mouse antibody
  • Anti-CSK (5E10) Mouse antibody

Anti-CSK (5E10) Mouse antibody

Cat.#: 168292

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Product Information

  • Product Name
    Anti-CSK (5E10) Mouse antibody
  • Documents
  • Description
    CSK (5E10) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, FC
  • Species reactivity
    Human, Mouse, Monkey, Rat
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human CSK expressed in E. Coli.
  • Clonality
    Monoclonal antibody
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot analysis of extracts from Ramos,NTERA2,COS7,Jurkat and Hela cell lysates using Csk mouse mAb (1:1000 diluted). Predicted band size:50KDa. Observed band size:50KDa.

    Western blot analysis of extracts from Ramos,NTERA2,COS7,Jurkat and Hela cell lysates using Csk mouse mAb (1:1000 diluted). Predicted band size:50KDa. Observed band size:50KDa.

    Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).

    Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).

    Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Background
    Swiss-Prot Acc.P41240.Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"