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Product Name
CLTB antibody
- Documents
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Description
CLTB Mouse Monoclonal antibody. Positive WB detected in pig brain tissue. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue, human kidney tissue. Observed molecular weight by Western-blot: 32-38 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Pig; other species not tested.
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Alternative names
Clathrin light chain B antibody; clathrin antibody; light chain (Lcb) antibody; CLTB antibody; LCB antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of CLTB recombinant protein (Accession Number: NM_001834). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
IF: 1:50-1:500
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Validations
pig brain tissue were subjected to SDS PAGE followed by western blot with (CLTB Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107163(CLTB Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107163(CLTB Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:107163(CLTB Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"