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Product Name
CLTB antibody
- Documents
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Description
CLTB Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, human brain tissue, mouse brain tissue, PC-3 cells. Positive IP detected in mouse brain tissue. Positive IF detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 32-38 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Clathrin light chain B antibody; clathrin antibody; light chain (Lcb) antibody; CLTB antibody; LCB antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLTB recombinant protein (Accession Number: NM_001834). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:109406(CLTB antibody) at dilution of 1:1000
IP Result of anti-CLTB (IP:Catalog No:109406, 4ug; Detection:Catalog No:109406 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109406(CLTB Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
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