CHOP antibody

Cat.#: 109271

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Product Information

  • Product Name
    CHOP antibody
  • Documents
  • Description
    CHOP Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue. Positive WB detected in RAW264.7 cells, A375 cells, HeLa cells, MCF7 cells, Tunicamycin treated HeLa cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, IHC, FC, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CEBPZ antibody; CHOP antibody; CHOP 10 antibody; DDIT 3 antibody; DDIT3 antibody; GADD153 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CHOP recombinant protein (Accession Number: NM_004083). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109271(CHOP; GADD153 antibody) at dilution of 1:1000

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:109271(CHOP; GADD153 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109271(CHOP; GADD153 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109271(CHOP; GADD153 antibody) at dilution of 1:50 (under 40x lens)

    Tunicamycin treated Hela cells were subjected to SDS PAGE followed by western blot with Catalog No:109271(CHOP; GADD153 Antibody) at dilution of 1:300

    Tunicamycin treated Hela cells were subjected to SDS PAGE followed by western blot with Catalog No:109271(CHOP; GADD153 Antibody) at dilution of 1:300

    1X10^6 HeLa cells were stained with 0.2ug CHOP; GADD153 antibody (Catalog No:109271, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug CHOP; GADD153 antibody (Catalog No:109271, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions to hamster gene.Imposed by unfolded and malfolded proteins, CHOP is significantly induced by ER stress, deficiency of CHOP prevents cell from ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP by forming a heterodimer. It may play an important role in the malignant transformation of nevus to melanoma. The observed molecular weight of CHOP is about 30 kDa.
  • References
    • Li K, Zhang L, Xiang X. Arsenic trioxide alleviates airway hyperresponsiveness and promotes apoptosis of CD4+ T lymphocytes: evidence for involvement of the ER stress-CHOP pathway. Irish journal of medical science. 182(4):573-83. 2013.
    • Sun L, Liu N, Liu SS. Beclin-1-independent autophagy mediates programmed cancer cell death through interplays with endoplasmic reticulum and/or mitochondria in colbat chloride-induced hypoxia. American journal of cancer research. 5(9):2626-42. 2015.
    • Huang M, Xu A, Wu X. Japanese encephalitis virus induces apoptosis by the IRE1/JNK pathway of ER stress response in BHK-21 cells. Archives of virology. 161(3):699-703. 2016.
    • Lei X, Cui K, Liu Q. Exogenous Estradiol Benzoate Induces Spermatogenesis Disorder through Influencing Apoptosis and Oestrogen Receptor Signalling Pathway. Reproduction in domestic animals = Zuchthygiene. 51(1):75-84. 2016.
    • Li J, Liu K, Liu Y. Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Nature immunology. 14(8):793-803. 2013.
    • Xue J, Wei J, Dong X. ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway. The journal of physiological sciences : JPS. 63(6):435-44. 2013.
    • Wang SQ, Niu XL, Liu ZW, Zhu YH, Gao DF. Selenium deficiency is associated with endoplasmic reticulum stress in a rat model of cardiac malfunction. Biological trace element research. 156(1-3):196-201. 2013.
    • Liu ZW, Zhu HT, Chen KL. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) signaling pathway plays a major role in reactive oxygen species (ROS)-mediated endoplasmic reticulum stress-induced apoptosis in diabetic cardiomyopathy. Cardiovascular diabetology. 12:158. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"