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Product Name
CHD3 Polyclonal Antibody
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Description
Polyclonal antibody to CHD3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHD3 antibody; Mi-2a antibody; Mi2-ALPHA antibody; ZFH antibody; chromodomain-helicase-DNA-binding protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human CHD3 (NP_001005273.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:1000
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Validations
Western blot - CHD3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHD3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
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Background
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"