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Product Name
CHD3 Monoclonal Antibody
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Description
Monoclonal antibody to CHD3
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Tested applications
WB, IHC, FC
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Species reactivity
Human
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Alternative names
CHD3 antibody; Mi-2a antibody; Mi2-ALPHA antibody; ZFH antibody; chromodomain-helicase-DNA-binding protein 3 antibody
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Isotype
Mouse IgG
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Preparation
Antigen: Recombinant protein of human CHD3
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
FC 1:20 - 1:50 -
Validations
Western blot - CHD3 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using CHD3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.
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Background
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"