Anti-CHD3 (7F6) Mouse antibody

Cat.#: 168252

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Product Information

  • Product Name
    Anti-CHD3 (7F6) Mouse antibody
  • Documents
  • Description
    CHD3 (7F6) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-a antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascites containing with 0.03% Proclin300 and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/400

  • Validations

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).

    Western blot detection of CHD3 (C-terminus) in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.

    Western blot detection of CHD3 (C-terminus) in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.

    Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.

  • Background
    Swiss-Prot Acc.Q12873.Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"