-
Product Name
CDC42 antibody
- Documents
-
Description
CDC42 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human gliomas tissue, human pancreas tissue, human skeletal muscle tissue. Positive IF detected in C6 cells, HeLa cells. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 21 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CDC42 antibody; CDC42Hs antibody; G25K antibody; G25K GTP binding protein antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CDC42 recombinant protein (Accession Number: NM_001791). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109105(CDC42 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109105(CDC42 antibody) at dilution of 1:50 (under 25x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed C6 cells using Catalog No:109105(CDC42 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.
-
References
- Ju J, Qi Z, Cai X. Toosendanin induces apoptosis through suppression of JNK signaling pathway in HL-60 cells. Toxicology in vitro : an international journal published in association with BIBRA. 27(1):232-8. 2013.
- Zallocchi M, Johnson BM, Meehan DT, Delimont D, Cosgrove D. α1β1 integrin/Rac1-dependent mesangial invasion of glomerular capillaries in Alport syndrome. The American journal of pathology. 183(4):1269-80. 2013.
- Deng Z, Huang Z, Yuan M, Yang K, Pang Y. Baculovirus induces host cell aggregation via a Rho/Rok-dependent mechanism. The Journal of general virology. 95(Pt 10):2310-20. 2014.
- Zhou T, Zhang B, Wei P. Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets. Biomaterials. 35(37):9833-43. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"