Human CD40L/CD154/TNFSF5 (Fc Tag) recombinant protein

Human CD40L/CD154/TNFSF5 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.

CD40 ligand

CD40L; TRAP; CD40-L; RP23-153G22.3; RP3-527F8.3; GP39; TNFSF5; LY-62; HIGM1; IGM; T-BAM; CD40LG; IMD3; HCD40L; CD154; LY62

P29965

CD40LG; CD40L; TNFSF5; TRAP

Human Cells

A DNA sequence encoding the soluble form of human CD40L (NP_000065.1) (Met 113-Leu 261) was fused with the Fc region of human IgG1 at the N-terminus.

Human

The recombinant human CD40L/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 42.8 kDa. The apparent molecular mass of rh CD40L/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 μg/ml (100 μl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.