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Product Name
Anti-CD40L/CD154/TNFSF5 antibody
- Documents
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Description
Rabbit polyclonal to CD40L/CD154/TNFSF5
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Tested applications
ELISA, WB
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Species reactivity
Human CD40L / CD154 / TNFSF5
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Alternative names
CD154 antibody; CD154 antibody; Cd40l antibody; CD40L antibody; Cd40lg antibody; CD40LG antibody; gp39 antibody; gp39 antibody; hCD40L antibody; HIGM1 antibody; HIGM1 antibody; IGM antibody; IGM antibody; IMD3 antibody; IMD3 antibody; Ly62 antibody; Ly-62 antibody; RP23-153G22.3 antibody; RP3-527F8.3 antibody; T-BAM antibody; Tnfsf5 antibody; TNFSF5 antibody; TRAP antibody; TRAP antibody; T-BAM antibody; IGM antibody; GP39 antibody; IMD3 antibody; TRAP antibody; HIGM1 antibody; T-BAM antibody; IGM antibody; IMD3 antibody; TRAP antibody; gp39 antibody; CD154 antibody; CD40L antibody; HIGM1 antibody; T-BAM antibody; TNFSF5 antibody; hCD40L antibody; Ly62 antibody; CD154 antibody; Cd40l antibody; Ly-62 antibody; CD40-L antibody; Tnfsf5 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CD40L / CD154 / TNFSF5 (rh CD40L / CD154 / TNFSF5; NP_000065.1; Glu108-Leu261). CD40L / CD154 / TNFSF5 specific IgG was purified by Human CD40L / CD154 / TNFSF5 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-20 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human CD40L / CD154 / TNFSF5.
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Validations
CD40L / CD154 / TNFSF5 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
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References
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
- Grewal IS, et al. (1998) CD40 and CD154 in cell-mediated immunity. Annual Review of Immunology. 16: 111-35.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"