Cynomolgus CTLA-4/CD152 (Fc Tag) recombinant protein

Cynomolgus CTLA-4/CD152 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

CD152 protein

CTLA4; CD; LY-56; CH29-120A16.2; ICOS; ALPS5; IDDM12; GSE; CTLA-4; CD152; CELIAC3; GRD4

Q9BDC4

CTLA-4; CTLA4; EGK_04712

Human Cells

A DNA sequence encoding the cynomolgus/rhesus CTLA4 (Q9BDC4) (Met1-Asp161) was expressed with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus CTLA4 sequences are identical.

Cynomolgus

The recombinant cynomolgus/rhesus CTLA4 is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a calculated molecular mass of 40.4 KDa.The apparent molecular mass of the protein is approximately 52 KDa respectively in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human B7-1-His (Cat:503898) at 10 μg/ml (100 μl/well) can bind Cynomolgus CTLA4-Fc (Cat:502754), The EC₅₀ of Cynomolgus CTLA4-Fc is 6.0-14.5 ng/ml.