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Product Name
CBS antibody
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Description
CBS Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in mouse kidney tissue, HeLa cells, mouse brain tissue, rat brain tissue, rat liver tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 61kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Beta thionase antibody; CBS antibody; cystathionine beta synthase antibody; HIP4 antibody; Serine sulfhydrase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CBS recombinant protein (Accession Number: NM_001178009). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:108942(CBS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108942(CBS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108942(CBS antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CBS antibody Catalog No:108942 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CBS (IP:Catalog No:108942, 3ug; Detection:Catalog No:108942 1:500) with mouse kidney tissue lysate 4000ug.
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Background
The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).
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References
- Stipanuk MH, Ueki I. Dealing with methionine/homocysteine sulfur: cysteine metabolism to taurine and inorganic sulfur. Journal of inherited metabolic disease. 34(1):17-32. 2011.
- Zhang G, Wang P, Yang G, Cao Q, Wang R. The inhibitory role of hydrogen sulfide in airway hyperresponsiveness and inflammation in a mouse model of asthma. The American journal of pathology. 182(4):1188-95. 2013.
- Roman HB, Hirschberger LL, Krijt J, Valli A, Kožich V, Stipanuk MH. The cysteine dioxgenase knockout mouse: altered cysteine metabolism in nonhepatic tissues leads to excess H2S/HS(-) production and evidence of pancreatic and lung toxicity. Antioxidants & redox signaling. 19(12):1321-36. 2013.
- Jurkowska H, Roman HB, Hirschberger LL. Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate. Amino acids. 46(5):1353-65. 2014.
- Salloum FN, Sturz GR, Yin C. Beetroot juice reduces infarct size and improves cardiac function following ischemia-reperfusion injury: Possible involvement of endogenous H2S. Experimental biology and medicine (Maywood, N.J.). 240(5):669-81. 2015.
- Nalli AD, Rajagopal S, Mahavadi S, Grider JR, Murthy KS. Inhibition of RhoA-dependent pathway and contraction by endogenous hydrogen sulfide in rabbit gastric smooth muscle cells. American journal of physiology. Cell physiology. 308(6):C485-95. 2015.
- Brown-Borg HM, Rakoczy S, Wonderlich JA, Armstrong V, Rojanathammanee L. Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice. Longevity & healthspan. 3(1):10. 2014.
- Miyamoto R, Otsuguro K, Yamaguchi S, Ito S. Neuronal regulation of expression of hydrogen sulfide-producing enzyme cystathionine β-synthase in rat spinal cord astrocytes. Neuroscience research. 97:52-9. 2015.
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