Mouse beta-Catenin/CTNNB1 (His & GST Tag) recombinant protein

Mouse beta-Catenin/CTNNB1 (His & GST Tag) recombinant protein

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Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (By similarity). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (PubMed:21325504).

Catenin beta-1

CTNNB; CATENIN BETA-1; ARMADILLO; CTNNB1; FLJ37923; BETA-CATENIN; BFC; CATNB; DKFZP686D02253; FLJ25606; OK/SW-CL.35; MESC; MRD19

Q02248

Ctnnb1; Catnb

Baculovirus-Insect Cells

A DNA sequence encoding the mouse CTNNB1 (Q02248) (Met1-Leu781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Mouse

The recombinant mouse CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. The recombinant protein migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE