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Product Name
Anti-Phospho-beta Catenin (Ser33) Rabbit antibody
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Description
Phospho-beta Catenin (Ser33) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human β-Catenin.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000IHC:1/50-1/100
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Validations
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using u03b2-Catenin (Phospho-Ser33) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot detection of u03b2-Catenin(Phospho-Ser33) in Hela cells untreated or Calyculin A-treated, using u03b2-Catenin(Phospho-Ser33) Rabbit pAb (dilution 1:1000, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:92kDa.Observed band size:92kDa.
Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using u03b2-Catenin (Phospho-Ser33) Antibody .
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Background
Swiss-Prot Acc.P35222.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"