Human Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein

Human Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Carbonic anhydrase 9

CA9; CARBONIC ANHYDRASE IX; CAIX; MN/CA9; CAR9; CARBONIC ANHYDRASE 9; MN; RP23-195K8.9

Q16790

CA9; G250; MN

Human Cells

A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human CA9 consists of 388 amino acids and predicts a molecular mass of 42.5 kDa. As a result of glycosylation, rhCA9 migrates with apparent molecular mass of 48 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its esterase activity. The specific activity is >30 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.