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Product Name
Anti-Carbonic Anhydrase IX/CA9 antibody
- Documents
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Description
Rabbit polyclonal to Carbonic Anhydrase IX/CA9
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Tested applications
ELISA, WB, IP
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Species reactivity
Canine Carbonic Anhydrase IX / CA9
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Alternative names
Car9 antibody; Carbonic Anhydrase 9 antibody; Carbonic Anhydrase IX antibody; Carbonic Anhydrase IX antibody; MN/CA9 antibody; RP23-195K8.9 antibody; MN antibody; CA9 antibody; CAIX antibody; CAIX antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Canine Carbonic Anhydrase IX / CA9 (rh Carbonic Anhydrase IX / CA9; NP_001138646.1; Met1-Leu410). Carbonic Anhydrase IX / CA9 specific IgG was purified by Canine Carbonic Anhydrase IX / CA9 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-50 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Canine Carbonic Anhydrase IX / CA9. The detection limit for Canine Carbonic Anhydrase IX / CA9 is < 0.039 ng/well.
IP: 4-6 μg/mg of lysate
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Validations
Carbonic Anhydrase IX / CA9 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Carbonic Anhydrase IX / CA9 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
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References
- Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9.
- Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71.
- Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27.
- Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"