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Product Name
CA IX antibody
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Description
CA IX Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CA IX antibody; CA9 antibody; CAIX antibody; Carbonate dehydratase IX antibody; Carbonic anhydrase 9 antibody; carbonic anhydrase IX antibody; G250 antibody; Membrane antigen MN antibody; P54/58N antibody; pMW1 antibody; RCC assOCiated antigen G250 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CA IX recombinant protein (Accession Number: NM_001216). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108808(CA9 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:108808(CA9 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:108808(CA9 Antibody) at dilution of 1:50 (under 40x lens)
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Background
CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549(Joc,vol21,no.3,473-482).
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