Human BMPR2 (His & Fc Tag) recombinant protein

Human BMPR2 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The bone morphogenetic protein type II receptor (BMPR-II, or BMPR2), a receptor for the transforming growth factor (TGF)-beta/bone morphogenetic protein (BMP) superfamily. Reduced expression or function of BMPR2 signaling leads to exaggerated TGF-beta signaling and altered cellular responses to TGF-beta. In endothelial cells, BMPR2 mutation increases the susceptibility of cells to apoptosis. BMPR2 transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. The intracellular domain of BMPR2 is both necessary and sufficient for receptor complex interaction. It had been identified that BMPR2 plays a key role in cell growth. Its mutations lead to hereditary pulmonary hypertension, and knockout of Bmpr-II results in early embryonic lethality. The C-terminal tail of BMPR2 provides binding sites for a number of regulatory proteins that may initiate Smad-independent signalling. BMPR2 mutations were predicted to alter the BMP and TGF-b1/SMAD signalling pathways, resulting in proliferation rather than apoptosis of vascular cells, and greatly increase the risk of developing severe pulmonary arterial hypertension. BMPR2 gene result in familial Primary pulmonary hypertension (PPH) transmitted as an autosomal dominant trait, albeit with low penetrance. Heterozygous germline mutations of BMPR2 gene have been identified in patients with familial and sporadic PPH, indicating that BMPR2 may contribute to the maintenance of normal pulmonary vascular structure and function. Tctex-1, a light chain of the motor complex dynein, interacts with the cytoplasmic domain of BMPR2 and demonstrate that Tctex-1 is phosphorylated by BMPR-II, a function disrupted by PPH disease causing mutations within exon 12. BMPR2 and Tctex-1 co-localize to endothelium and smooth muscle within the media of pulmonary arterioles, key sites of vascular remodelling in PPH.

Bone morphogenetic protein receptor type-2

GM20272; FLJ41585; BMPR3; 2610024H22RIK; FLJ76945; BMPR-2; BRK-3; BMR2; BMPR2; T-ALK; POVD1; BMPR-II; PPH1; BMPRII; BB189135; AW546137; BMP-2; AL117858

Q13873

BMPR2; PPH1

Human Cells

A DNA sequence encoding the human BMPR-II (NP_001195.2) extracellular domain (Met 1-Ile 151) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human BMPR-II/Fc is a disulfide-linked homodimer. The reduced monomer consists of 373 amino acids and has a predicted molecular mass of 42 kDa. As a result of glycosylation, the apparent molecular mass of rh BMPR-II/Fc monomer migrates with an apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human BMPR-II-Fc (Cat:502741) at 10 μg/mL (100 μl/well) can bind  biotinylated human BMP2-Fc (Cat:500392), The EC₅₀ of biotinylated human BMP2-Fc (Cat:500392) is 80-110 ng/mL.