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Product Name
BMPR2 antibody
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Description
BMPR2 Rabbit Polyclonal antibody. Positive FC detected in PC-3 cells. Positive WB detected in human heart tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BMP type 2 receptor antibody; BMP type II receptor antibody; BMPR 2 antibody; BMPR II antibody; BMPR2 antibody; BMPR3 antibody; BMR2 antibody; BRK 3 antibody; FLJ41585 antibody; FLJ76945 antibody; PPH1 antibody; T ALK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BMPR2 recombinant protein (Accession Number: NM_001204). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:117204(BMPR2 antibody) at dilution of 1:400
IP Result of anti-BMPR2 (IP:Catalog No:117204, 4ug; Detection:Catalog No:117204 1:1500) with mouse brain tissue lysate 4000ug.
1X10^6 PC-3 cells were stained with 0.2ug BMPR2 antibody (Catalog No:117204, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. Dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form and 150 kD dimer.
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