Anti-UBE2M antibody

Cat.#: 106285

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Product Information

  • Product Name
    Anti-UBE2M antibody
  • Documents
  • Description
    Rabbit polyclonal to UBE2M
  • Tested applications
  • Species reactivity
    Human UBE2M
  • Alternative names
    UBC12 antibody; UBC12 antibody; UBC-RS2 antibody; UBC12 antibody; hUbc12 antibody; UBC-RS2 antibody; UBC12 antibody; Ubc-rs2 antibody; 2510040H03Rik antibody; 2510040H03Rik antibody; hUbc12 antibody; Ubc-rs2 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Human UBE2M (rh UBE2M; P61081; Met1-Lys183). UBE2M specific IgG was purified by Human UBE2M affinity chromatography.
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 1-10 μg/ml

    ELISA: 0.1-0.2 μg/ml

    This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human UBE2M.

    IP: 1-4 uL/mg of lysate

  • Validations

    UBE2M Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    UBE2M Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

  • Background
    UBE2M is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 
  • References
    • Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317.
    • Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.