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Product Name
UBC12 antibody
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Description
UBC12 Rabbit Polyclonal antibody. Positive IF detected in L02 cells. Positive WB detected in L02 cells, SMMC-7721 cells. Positive IP detected in L02 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hUbc12 antibody; NEDD8 carrier protein antibody; NEDD8 conjugating enzyme Ubc12 antibody; NEDD8 protein ligase antibody; UBC RS2 antibody; UBC12 antibody; UBE2M antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBC12 recombinant protein (Accession Number: NM_003969). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116476(UBC12 Antibody) at dilution of 1:600
IP Result of anti-UBC12 (IP:Catalog No:116476, 4ug; Detection:Catalog No:116476 1:600) with L02 cells lysate 1800ug.
Immunofluorescent analysis of L02 cells using Catalog No:116476(UBC12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
UBE2M represents ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"