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Product Name
Anti-TSPAN1 antibody
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Description
Rabbit monoclonal to TSPAN1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human TSPAN1
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Alternative names
9030418M05Rik antibody; NET1 antibody; RP23-319H15.4 antibody; tetraspan 1 antibody; tetraspan 1 antibody; tetraspanin 1 antibody; tetraspanin 1 antibody; TM4C antibody; TM4SF antibody; TSPAN1 antibody; Tspan1 antibody; tspan-1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TSPAN1 (rh TSPAN1; O60635; Tyr110-Asn211).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human TSPAN1. The detection limit for Human TSPAN1 is approximately 0.00975 ng/well.
IHC-P: 0.05-3 μg/mL
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Validations
TSPAN1 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human TSPAN1 in human prostate with rabbit monoclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
TSPAN1 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human TSPAN1 in human rectal cancer with rabbit monoclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
TSPAN1 belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins have four hydrophobic domains, intracellular N- and C-termini and two extracellular domains. Tetraspanins act as scaffolding proteins, anchoring multiple proteins to one area of the cell membrane. They also mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN1 interacts with human thiamine transporter-1 (hTHTR-1). HTHTR-1 contributes to intestinal thiamine uptake, and its function is regulated at both the transcriptional and posttranscriptional levels. TSPAN1 and hTHTR-1 colocalize in human intestinal epithelial HuTu-80 cells. Coexpression of TSPAN1 in these cells led to a significant decrease in the rate of degradation of hTHTR-1 compared with cells expressing the hTHTR-1 alone; in fact the half-life of the TSPAN1 protein was twice longer in the former cell type compared with the latter cell type.
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References
- Chen L. et al., 2010, J Korean Med Sci. 25 (10): 1438-42.
- Chen L. et al., 2010, Tumori. 96 (5): 744-50.
- Nabokina SM. et al., 2011, Am J Physiol Gastrointest Liver Physiol. 301 (5): G808-13.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"