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Product Name
Anti-Src Rabbit antibody
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Description
Src Rabbit polyclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from Human Src.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/1000IHC:1/50-1/100
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Validations
Western blot detection of Src (Ab-529) in Hela,C6,3T3 and PC-12 cell lysates using Src (Ab-529) rabbit pAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Ab-529) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HT29, Hela and MDA cells using Src (Ab-529) Antibody .
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Background
Swiss-Prot Acc.P12931.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"