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Product Name
Anti-Phospho-Src (Tyr530) Rabbit antibody
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Description
Phospho-Src (Tyr530) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529) Antibody .
Western blot analysis of extracts from COS7 and 3T3 cells, using SRC(Phospho-Tyr529) Rabbit mAb.The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.
Immunofluorescence staining of methanol-fixed Hela cells using Src (Phospho-Tyr529) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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Background
Swiss-Prot Acc.P12931.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"