Anti-RPA32/RPA2 Rabbit antibody
RPA32/RPA2 Rabbit monoclonal antibody
WB, IHC-P, IP
Human, Mouse, Rat
REPA2; RPA32; RP-A p32; RP-A p34 antibody
Antigen: A synthetic peptide of human RPA32/RPA2
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Western blot detection of RPA32/RPA2 in Hela,CHO-K1,C6,Jurkat cell lysates using RPA32/RPA2 Rabbit mAb(1:500 diluted).Predicted band size:29kDa.Observed band size:29kDa.
Swiss-Prot Acc.P15927.As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
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