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Product Name
Anti-RPA32/RPA2 (3E7) Mouse antibody
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Description
RPA32/RPA2 (3E7) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human
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Alternative names
60S acidic ribosomal protein P1;AA409079;AI325195;AU020965;HSSB;ik:tdsubc_2g1;M(2)21C; MGC137236;OTTHUMP00000004008;p32;p34;RCJMB04_6d17 replication protein A2 antibody; 32kDa;REPA 2; REPA1;REPA2;Replication factor A protein 2;Replication protein A 32 kDa subunit; antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000
;ICC: 1/200
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Validations
Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using anti-RPA32/RPA2 antibody (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using RPA32/RPA2 mouse mAb.
Western blot detection of RPA32/RPA2 in MCF7,Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s.
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Background
Swiss-Prot Acc.P15927.As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"