-
Product Name
Anti-Rb (7H10) Mouse antibody
- Documents
-
Description
Rb (7H10) Mouse monoclonal antibody
-
Tested applications
WB, ICC/IF, FC
-
Species reactivity
Human
-
Isotype
Mouse IgG1
-
Preparation
Antigen: Purified recombinant fragment of human RB1 (AA: 2106-2784) expressed in E. Coli.
-
Clonality
Monoclonal
-
Formulation
Purified antibody in PBS with 0.05% sodium azide
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
-
Validations
Flow cytometric analysis of Jurkat cells using RB1 mouse mAb (green) and negative control (red).
Western blot detection of Rb in Jurkat and MCF7 cell lysates using Rb mouse mAb (1:2000 diluted).Predicted band size:106KDa.Observed band size:110KDa.
Immunofluorescence analysis of NIH/3T3 cells using RB1 mouse mAb (green). Blue
-
Background
Swiss-Prot Acc.P06400.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"