-
Product Name
Anti-Phospho-Rb (Ser780) Rabbit antibody
- Documents
-
Description
Phospho-Rb (Ser780) Rabbit polyclonal antibody
-
Tested applications
WB, IHC-P
-
Species reactivity
Human
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthesized peptide derived from human Retinoblastoma
-
Clonality
Polyclonal
-
Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
-
Validations
Western blot analysis of Retinoblastoma phosphorylation expression in Jurkat cell lysate treated with Alkaline Phosphatase.
-
Background
Swiss-Prot Acc.P06400.Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"