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Product Name
Anti-PUMA Rabbit antibody
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Description
PUMA Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human
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Alternative names
JFY1; PUMA; JFY-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PUMA
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20-1/100
ICC/IF: 1/50-1/100
FC: 1/20
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Validations
Immunohistochemistry of PUMA in paraffin-embedded Human breast cancer tissue using PUMA Rabbit pAb at dilution 1/20
Western blot detection of PUMA in K562 cell lysates using PUMA Rabbit pAb(1:1000 diluted).Predicted band size:21KDa.Observed band size:21KDa.
Western blot detection of PUMA in Jurkat,THP-1,C6,3T3,Hela cell lysates using PUMA Rabbit pAb(1:1000 diluted).Predicted band size:21KDa.Observed band size:21KDa.
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Background
Swiss-Prot Acc.Q9BXH1.Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391). Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis (PubMed:23340338).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"