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Product Name
Anti-PUMA Rabbit antibody
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Description
PUMA Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human PUMA
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/50
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Validations
Immunofluorescent analysis of Hela cells, using PUMA Antibody .
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PUMA Antibody.
Western blot analysis of PUMA expression in Hela,THP-1,K562 and HL60 cell lysates using PUMA antibody at 1/1000 dilution.Predicted band size:18KDa.Observed band size:18KDa.
Western blot analysis of PUMA expression in K562 cell lysate.
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Background
Swiss-Prot Acc.Q9BXH1.Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"