Cart 2

Anti-PI3 Kinase p85 alpha (9D2) Mouse antibody

Cat.#: 168502

Size:

Special Price 441.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-PI3 Kinase p85 alpha (9D2) Mouse antibody
  • Documents
  • Description
    PI3 Kinase p85 alpha (9D2) Mouse monoclonal antibody
  • Tested applications
    IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human PIK3R1 (AA: 159-388) expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified antibody in PBS with 0.05% sodium azide
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    IHC: 1/200 - 1/1000

    ICC: 1/100

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Immunofluorescence analysis of HeLa cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Immunofluorescence analysis of HeLa cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Flow cytometric analysis of NIH3T3 cells using PIK3R1 mouse mAb (green) and negative control (red).

    Flow cytometric analysis of NIH3T3 cells using PIK3R1 mouse mAb (green) and negative control (red).

    Immunofluorescence analysis of HepG2 cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Immunofluorescence analysis of HepG2 cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Western blot analysis using PIK3R1 mAb against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

    Western blot analysis using PIK3R1 mAb against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

    Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 mouse mAb with DAB staining.

  • Background
    Swiss-Prot Acc.P27986.Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"