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Product Name
Anti-Phospho-PI3 Kinase p85 (Tyr607) Rabbit antibody
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Description
Phospho-PI3 Kinase p85 (Tyr607) Rabbit polyclonal antibody
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Tested applications
ICC/IF, WB, IHC-P, ELISA
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Species reactivity
Human, Mouse, Rat, Chicken
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Isotype
Rabbit IgG
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Preparation
Antigen: The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607. AA range:573-622
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Clonality
Polyclonal
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Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
IF: 1/50-200
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of various cells using Phospho-PI 3-kinase p85α (Y607) Polyclonal Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-heart tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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Background
Swiss-Prot Acc.P27986.
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